Leader: Marek Basler
Co-leader: Jan-Willem Veening
Recent methodological advancements in ultra-sensitive analytics and single cell technologies are key for the acquisition of precise information about growth characteristics, physiological states and persistence phenotypes of bacteria in patients and in patient-like environments. This work package will establish advanced enabling technologies for the isolation and enrichment of specific pathogens and for the analysis of their specific physiology and genetic and proteome composition. This will include the development of enrichment techniques for specific pathogens and implementation of ultra-sensitive analysis tools such as proteomics, RNA-seq, metabolomics and flow cytometry. In addition, WP-TEC will establish a toolbox of chemical and biological probes to visualize at single cell level the bacterial physiology during growth arrest and antibiotic-induced killing in vitro and under patient-like conditions. Activities in WP-TEC are closely aligned with existing Core Facilities like the Genomics, FACS, Imaging, Proteomics, sciCORE Center for Scientific Computing, Single-cell, and BSL3 Facilities.